Figure 4.
Epitope-driven upregulation of CD107a on Lym-1 and CD19 CAR T cells. Lym-1 and CD19 CAR T cells were detected by protein L and APC-streptavidin flow cytometry. Mock transduced T cells were added to each preparation to adjust the CAR T cell fraction to 30%. T cells (2 × 105) were then incubated with 105 Raji or Daudi cells. Mock transduced T cells alone and CAR transduced T cells incubated with epitope-negative K562 cells served as negative controls. An anti-CD107a antibody and monensin were then added to the wells soon after. After a 5 h incubation, cells were labeled with PE-anti-CD3 antibody to differentiate tumor and T cells using flow cytometry. (Top panel) examples of data; (Bottom panel): data from n = 3 (ns, not significant; ** = p < 0.01; compared to CD107a level when co-incubated with K562).