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. 2017 Dec 20;18(12):2781. doi: 10.3390/ijms18122781

Figure 1.

Figure 1

In vivo and in vitro effects of leptin-AI and leptin-AII treatment on PRL transcripts in goldfish. Dose- (A) and time-dependent (B) effects of leptin-AI or leptin-AII IP injection on PRL mRNA expression in the pituitary. For time-course experiments, goldfish were IP injected with leptin-AI or leptin-AII at 100 ng/g for 0, 3, 6, 12, 24 and 48 h. For dose-dependence experiments, the goldfish were IP-injected with leptin-AI or leptin-AII for 24 h with increasing doses (1–100 ng/g). Dose- (C) and time-dependent (D) effects of leptin-AI or leptin-AII incubation on PRL mRNA expression in primary cultured pituitary cells. For time-course experiments, goldfish pituitary cells were incubated for 3, 6, 12, 24, and 48 h with leptin-AI or leptin-AII, at a concentration of 100 nM. For dose-dependence experiments, goldfish pituitary cells were incubated with leptin-AI or leptin-AII for 24 h with increasing doses (0.01–100 nM). In these studies, data are expressed as the mean ± standard error (SE, n = 10 for the in vivo study, and n = 4 for the in vitro study). The same letter represents a similar level of transcriptional expression (p > 0.05), and a different letter represents significant difference in levels of transcriptional expression between two groups (p < 0.05).