Skip to main content
. 2017 Dec 20;18(12):2781. doi: 10.3390/ijms18122781

Figure 4.

Figure 4

Effects of PI3K/Akt/mTOR signal pathway blockers on leptin-stimulated PRL mRNA levels in primary cultured goldfish pituitary cells. Goldfish pituitary cells were treated with leptin-AI (100 nM) or leptin-AII (100 nM) in the presence or absence of PI3K inhibitors LY294002 (10 μM, A) and wortmannin (100 nM, B), Akt inhibitor API2 (100 nM, C), and mTOR inhibitor rapamycin (20 nM, D) for 24 h. In the present study, the data are expressed as mean ± SE (n = 4). The same letter represents a similar level of transcriptional expression (p > 0.05), and a different letter represents significant difference in levels of transcriptional expression between two groups (p < 0.05).