. 2017 Dec 20;18(12):2781. doi: 10.3390/ijms18122781

Table 1.

Primers and amplification conditions for the qPCR analysis in this study.

Gene Target/Accession No.(Primer Sequences, 5’–3’)	PCR Condition			Cycle
Gene Target/Accession No.(Primer Sequences, 5’–3’)	Denaturing	Annealing	Extension	Cycle
**PRL/ S82197**
CACTCTCTCAGCACCTCTCTC	94 °C	58 °C	72 °C	× 35
CTCTTTGGTCTTGCTGTCAATG	30 s	30 s	30 s
**lepR/ EU911005**
TCATCAACCCAAACGACG	94 °C	56 °C	72 °C	× 35
GTGAACTCCTCTGAGCCATA	30 s	30 s	30 s
**leptin-AI / FJ534535**
TCCAAAGCTCCTCATAGG	94 °C	52 °C	72 °C	× 35
TGGTGGGTGGCGTTTTCC	30 s	30 s	30 s
**leptin-AII / FJ854572**
TATCGTGGACACCCTAACTAC	94 °C	52 °C	72 °C	× 35
GGTCTAAAGCCAAGAACCCTAA	30 s	30 s	30 s
GH/ EU157192
TTAACGACTTTGAGGACAGCCT	94 °C	58 °C	72 °C	× 35
CAGCTTCTCAGTGATCTGGTTG	30 s	30 s	30 s
**GTH-α/ AY800267**
GCTCCTGTCTATCAGTGTATG	94 °C	58 °C	72 °C	× 35
GCACCCGTTTAACTTCTTT	30 s	30 s	30 s
**SL-α/ EU580712**
ATATGTTTGTCCCGTACCCTCT	94 °C	56 °C	72 °C	× 35
TTTATCAGACACCCACTTGGTC	30 s	30 s	30 s
*SL-β/ CAU72940*
AGGGACCATGTGTTCTCCTAAA	94 °C	56 °C	72 °C	× 35
AGAACCAGTATACCCTGCTCCA	30 s	30 s	30 s
**POMC/ AJ431209**
AAGCGCTCCTACTCCATGGA	94 °C	60 °C	72 °C	× 35
CTCGTCCCAGGACTTCATGAA	30 s	30 s	30 s
**β-actin/ AB039726**
CTGGTATCGTGATGGACTCT	94 °C	56 °C	72 °C	× 35
AGCTCATAGCTCTTCTCCAG	30 s	30 s	30 s