Fig. 6.

miR-200c-3p was increased by starvation and an miR-200c-3p inhibitor blocked LC3-II expression during starvation. a The levels of miR-200c-3p during starvation. PC-3 cells were incubated in Hank’s balanced salt solution (HBSS) for 0–6 h and the levels of miR-200c-3p were determined by RT-qPCR. b Blocking of LC3-II expression in miR-200c-3p-transfected PC-3 cells during starvation. Two days after transfection with the control, miR-200c-3p inhibitor, or miR-200c-3p mimic, cells were incubated in HBSS medium for 0, 3, and 6 h. Western blotting was performed to determine the levels of LC3-II and β-actin. Data are presented as the mean ± SEM of triplicate samples. ***p < 0.001, **p < 0.01, and *p < 0.05