Table 1. Radiolabeling of FSC-ZEGFR:2377 and DFO-ZEGFR:2377 with Zirconium-89 (2.4 nmol, pH 7 at 28 °C and 85°C)a.
radiochemical
yield (RCY %) |
||||
---|---|---|---|---|
FSC-ZEGFR:2377 |
DFO-ZEGFR:2377 |
|||
time [min] | 28 °C | 85 °C | 28 °C | 85 °C |
10 | 33.7 ± 10.9 | 83.6 ± 7.9** | 37.9 ± 20.6 | 96.5 ± 3.0* |
30 | 63.2 ± 11.6 | 96.8 ± 1.8** | 50.2 ± 16.3 | 97.5 ± 1.4* |
45 | n.d | n.d | 62.3 ± 14.4 | 98.2 ± 1.0* |
60 | 82.7 ± 10.3 | 97.7 ± 0.1 | 74.4 ± 9.9 | 98.5 ± 0.6* |
90 | 88.1 ± 7.4 | 98.6 ± 1.0 | 81.8 ± 0.2 | n.d |
120 | 91.9 ± 5.8 | n.d | 83.1 ± 2.6 | n.d |
Data were calculated from ITLC measurement of the nonpurified bioconjugates and are presented as mean ± standard deviation (n = 4), statistical analysis was performed using the Student’s t test with P values indicating very significant (** P < 0.01) and significant (* P < 0.05) difference between various temperature.