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. Author manuscript; available in PMC: 2018 Jan 3.
Published in final edited form as: ACS Catal. 2017 Aug 9;7(9):6352–6364. doi: 10.1021/acscatal.7b01861

Figure 1.

Figure 1

Biochemical characterization of ElDyP. (A) SEC chromatography of wt-ElDyP (red) and molecular weight standards (black) loaded onto a GE Superdex 200 Increase 10/300 GL column eluted with 50 mM potassium phosphate buffer (pH 7.5) containing 150 mM NaCl. The inset represents a MW calibration curve. (B) Changes in the UV-Vis absorption spectrum showing stepwise oxidation of Fe2+−wt-ElDyP (red) to Fe3+−wt-ElDyP (blue). The inset represents three individual spectroelectrochemical titrations of wt-ElDyP monitored by ΔA434 nm. (C) Normalized UV-Vis absorption spectra of wt and mutant ElDyPs with 450–700 nm region magnified by 4 times. (D) pH-activity profiles of wt and mutant ElDyPs with ABTS.