Table 1.

Summary of enzyme steady-state and transient-state kinetics

	RB19 (H2O2 at 1 mM)				H2O2 (RB19 at 100 μM)			Compound I
TcDyPs	kcat (s−1)	KM (μM)	kcat/KM (M−1 s−1)	ha	kcat (s−1)	KM (μM)	kcat/KM (M−1 s−1)	kobsb (M−1 s−1)	kdc (s−1)
wt	96 ± 2	42 ± 1	2.3×106	2.9	96 ± 2	188 ± 13	5.1×105	4.2×106	2.10
W263F	49 ± 1	33 ± 1	1.5×106	3.4	60 ± 1	219 ± 17	2.7×105	3.5×106	0.71
W263S	51 ± 1	32 ± 1	1.6×106	2.9	47 ± 1	178 ± 13	2.4×105	3.1×106	0.62
W263A	52 ± 1	28 ± 1	1.9×106	3.5	63 ± 2	303 ± 25	2.0 ×105	2.6×106	0.82
W376F	119 ± 5	49 ± 2	2.4×106	2.5	133 ± 3	295 ± 18	4.5×105	2.9×106	0.19
W376F/W263F	69 ± 1	26 ± 1	2.7×106	2.7	90 ± 3	283 ± 23	3.2×105	2.9×106	0.17
Y332F	83 ± 1	34 ± 1	2.5×106	3.4	108 ± 4	393 ± 32	2.7×105	3.4×106	1.90
Y332L	97 ± 1	37 ± 1	2.5×106	3.4	118 ± 2	279 ± 13	4.2×105	3.2×106	1.80

^aHill constant, h;

^bSecond -order rate constants (k_obs) of compound I formation in the presence of H₂O₂;

^cRate constants (k_d) of compound I decaying to compound II-like product when the reaction was performed with 5 μM enzyme and 5 μM H₂O₂ at pH 7.8.