Fig. 4.

Effect of E₂ on proliferation during acute inflammation. Two hours prior to kill, mice were injected with 5-bromo-2′-deoxyuridine (BrdU). Immunohistochemistry for BrdU was performed on sectioned tissues from the proximal and distal colon. Data are expressed as the percentage of proliferative cells compared to the total number of cells for either the full crypt column or the indicated region of the crypt. Data are representative of 20 well-oriented crypts per animal per proximal or distal colon with n = 12–16 mice per group. a Proliferation in the proximal colon and b proliferation in the distal colon. “*” denotes significance between indicated groups, P < 0.05