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. 2017 Nov 26;10:170–186. doi: 10.1016/j.omtn.2017.11.011

Figure 3.

Figure 3

Overexpression of miR-539-5p Inhibited VM Formation

(A) Relative expression levels of miR-539-5p in NBTs, LGGTs, and HGGTs. Data represent means ± SD (n = 3, NBT group; n = 10, each glioma tissue group). *p < 0.05 versus NBT group. #p < 0.05 versus LGGT group. (B) Relative expression levels of miR-539-5p in NHA, U87, and U251. Data represent means ± SD (n = 5, each). &p < 0.05 versus NHA group. (C) Relative miR-539-5p expression in U87 and U251 cells after transfection with miR-539-5p agomir and miR-539-5p antagomir. (D) CCK-8 assay was used to evaluate the proliferation effect of miR-539-5p on U87 and U251 cells. (E) Three-dimensional culture of U87 and U251 cells with the expression of miR-539-5p changed was calculated (magnification, 100×; scale bar, 100 μm). (F) Migration and invasion of U87 and U251 cells with the expression of miR-539-5p changed were measured (magnification, 200×; scale bar, 100 μm). (G) Western blot analysis was to evaluate the effect with the expression of miR-539-5p changed on the protein expression of MMP-2 and MMP-14 in U87 and U251 cells. Data represent means ± SD (n = 5, each). *p < 0.05 versus pre-NC group. #p < 0.05 versus anti-NC group. **p < 0.01 versus pre-NC group. ##p < 0.01 versus anti-NC group.