Figure 4.

LINC000339 Negatively Regulated and Targeted miR-539-5p

(A) Effect of knockdown of LINC00339 on the expression of miR-539-5p in U87 and U251 cells. Relative expression levels of miR-539-5p were detected by qRT-PCR. Data represent means ± SD (n = 3, each). *p < 0.05 versus shNC group. (B) Relative luciferase activity was performed by dual-luciferase reporter assay. Data represent means ± SD (n = 3, each). *p < 0.05 versus LINC00339-Wt + pre-NC group. (C) miR-539-5p was identified in LINC00339-RISC complex. LINC00339 and miR-539-5p expression levels were detected using qRT-PCR. Data represent means ± SD (n = 5, each). *p < 0.05 and **p < 0.01 versus anti-normal IgG group of respective group, ^#p < 0.05 and ^##p < 0.01 versus anti-Ago2 in control group.