Figure 5.

LINC00339 Regulated Tumor-Induced VM via Binding to miR-539-5p

(A) CCK-8 assay was used to evaluate the proliferation effect on U87 and U251 cells. (B) Three-dimensional culture of U87 and U251 cells was calculated (magnification, 100×; scale bar, 100 μm). (C) Migration and invasion of U87 and U251 cells were measured (magnification, 200×; scale bar, 100 μm). (D) Western blot analysis was to evaluate the effect on the protein expression of MMP-2 and MMP-14 in U87 and U251 cells. Data represent means ± SD (n = 5, each). *p < 0.05 versus shNC + pre-NC group. **p < 0.01 versus shNC + pre-NC group.