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miR-539-5p Targeted and Negatively Regulated TWIST1
(A) The protein expression of TWIST1 after cells were transfected with miR-539-5p agomir and miR-539-5p antagomir. Data represent means ± SD (n = 3, each). **p < 0.01 versus pre-NC group. ##p < 0.01 versus anti-NC group. (B) Relative luciferase activity was performed by dual-luciferase reporter assay. Data represent means ± SD (n = 3, each). *p < 0.05 versus TWIST1-Wt + pre-NC group.
