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. 2018 Jan 3;13(1):e0190523. doi: 10.1371/journal.pone.0190523

Fig 1. Survival of the double mutants before and after loss of Pot1 plasmid.

Fig 1

The pot1Δ cut17-275, pot1Δ bir1-T1, pot1Δ ark1-T7, pot1Δ ark1-T8 and pot1Δ pic1-T269 double mutants carrying plasmid-borne pot1+ and tk+ were streaked on selective and counter-selective media at the indicated temperatures. Pot1 plasmid was retained on EMM plates with adenine and uracil (EMM+AU). FUDR-containing plates were used as a counter selection to examine the ability of cells to grow after loss of the Pot1 plasmid.