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. 2018 Jan 3;13(1):e0190523. doi: 10.1371/journal.pone.0190523

Fig 3. Pic1 is required for the survival of trt1Δ cells having circular chromosomes.

Fig 3

(A) trt1Δ pic1-T269 cells were streaked on YEA+FUDR plates to select for cells that could grow after the loss of plasmid expressing trt1+ and tk+. The plasmid was retained on EMM plates supplemented with leucine and uracil (EMM+LU). (B) trt1Δ pic1-T269 double mutants lost telomeric DNA. The loss of telomeric DNA in trt1Δ pic1-T269 double mutant survivors was analyzed by Southern hybridization at 25°C. (C) NotI-digested chromosomal DNA from pic1-T269, trt1Δ and trt1Δ pic1-T269 cells were analyzed by PFGE at 25°C. (D) Lack of function of Pic1 results in loss of the viability of trt1Δ with circular chromosome. trt1Δ pic1-T269 double mutant cells having circular chromosomes were streaked on YEA plates at 33°C to examine the ability of the cells to grow. trt1Δ with circular chromosomes and pic1-T269 were used as controls.