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. Author manuscript; available in PMC: 2018 Nov 10.
Published in final edited form as: J Control Release. 2017 Aug 15;265:75–82. doi: 10.1016/j.jconrel.2017.08.012

Fig. 2. Immunotherapeutic schedule and serum analysis.

Fig. 2

(A) Preventive immunotherapy schedule. In brief mice were vaccinated on day 0, 7 and 14. After a rest period of four weeks, mice were sensitized twice at a weekly interval. Ten days post sensitization, mice were challenged consecutively for three days. (B) Anti-Ova IgG, IgG1, IgG2a and IgE response after vaccination (AV), after sensitization (AS) and after challenge (AC). Individual mouse serum was diluted to 1:500 for IgG, IgG1 and IgG2a analysis, and to 1:100 for IgE dilution. Error bars denote mean ± SEM. *: p < 0.05; ***: p < 0.0005, and ****: p < 0.0001.