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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Vaccine. 2017 Nov 7;35(51):7174–7180. doi: 10.1016/j.vaccine.2017.10.078

Figure 1. Anti-P. aeruginosa humoral immune responses after the immunization with AdC7OprF.RGD in mice with established P. aeruginosa respiratory infection.

Figure 1

C57BL/6 mice were intratracheally administrated with 1×106 cfu of P. aeruginosa embedded in agar beads and immunized intranasally with either 1×1010 particle units of AdC7OprF.RGD (PA+AdC7OprF.RGD), AdC7Null (PA+AdC7Null) or PBS (PA+PBS) 4 days after the administration of P. aeruginosa. Non-infected and non-immunized mice were used as controls (PBS). Anti-OprF antibody titers were determined in serum at 1 and 2 weeks after the immunization and in lung homogenate supernatants at 2 weeks after the immunization by ELISA. A. Serum anti-OprF IgG (1wk and 2 wk). B. Serum anti-OprF IgG isotypes (2 wk). C. Serum anti-OprF IgM (1 wk). D. Anti-OprF IgA in lung homogenate supernatants (2wk). Data are presented as mean ± SEM of n=7–8 mice per group. *, ** and ***denote significance of p < 0.05, p < 0.01 and p < 0.001 respectively.