Figure 2.

Cell viability was assessed by MTT assay. (A) Cells were treated with chlorogenic acid (0–500 µM) for 24 h. (B) Cells were treated with H₂O₂ (0–500 µM) for 24 h. (C) Effect of chlorogenic acid on HLE-B3 cell viability in co-treatment with H₂O₂ (100 µM). Blank cells were cultured in media alone, while control cells were treated with H₂O₂ (100 µM) alone. Data are presented as the mean + standard deviation. (^*P<0.05 and ^**P<0.01 vs. blank; ^#P<0.05 and ##P<0.01 vs. control; one-way analysis of variance and followed by Tukey's honest significant difference post hoc test).