Figure 2.

H₂O₂ induces apoptosis of MSCs. (A and B) Cells were treated with a range of H₂O₂ concentrations for 4 h, and apoptosis was analyzed by Annexin V-FITC/PI staining assay. (C) H₂O₂-induced cleaved caspase-3 expression was measured by western blotting, β-actin was used for normalization. Data are presented as the mean ± standard deviation of three independent measurements. ^$P<0.05 compared with the 0 µM H₂O₂ group. Cl.caspase-3, cleaved caspase-3; FITC, fluorescein isothiocyanate; H₂O₂, hydrogen peroxide; MSCs, mesenchymal stem cells; PI, propidium iodide.