Figure 3.

Evaluation of the binding activities of the identified proteins. (A) INS-1 cell nuclear extracts and purified fractions were subjected to immunoblotting with specific antibodies. Except for Sp3, 10% of the nuclear extract used for purification were loaded onto a gel. For Sp3, 50% of the nuclear extract was used for electrophoresis. (B) In vitro radiolabeled proteins were individually mixed with DNA-immobilized nanobeads of the single nucleotide polymorphism rs163184 region (risk or NR) or (−). Input and eluate fractions were separated using SDS-PAGE and visualized by autoradiography. Except for Sp3, 10% of the samples used for binding assay were loaded onto a gel. For Sp3, 50% of the sample was subjected to electrophoresis. NR, non-risk; (−), negative control nanobeads without immobilized DNA.