Figure 4.

Effects of the identified proteins on transcriptional activity under the control of the genomic region containing single nucleotide polymorphism rs163184. (A) Construction of Firefly luciferase reporter vectors. (B) 293T and HuH-7 cells were transfected with a Firefly luciferase reporter construct and a control Renilla luciferase reporter vector, in addition to a mammalian expression vector of individual identified proteins. (C) 293T cells were transfected with a Firefly luciferase reporter construct and a control Renilla luciferase reporter vector. At 12 h post-transfection, the medium was changed and inhibitors (2-PCPA or pargyline) were added (5-500 µM). ^*P<0.01 vs. the (−) group; ^#P<0.01 vs. the untreated group of each reporter construct. NR, non-risk; (−), without exogenous effectors.