Figure 5.

Allele-specific binding activity of Sp3 for the genomic region containing SNP rs163184. Cells were transfected with expression vectors for Sp3-Myc-FLAG. DNA-protein complexes were then immunoprecipitated with the anti-Myc-tag antibody. Enrichment of the target genomic regions was estimated by quantitative polymerase chain reaction analysis, and the fold enrichments of the SNP rs163184 region vs. the region 3,000 base pairs upstream and the region 3,000 base pairs downstream were determined. ^*P<0.01 vs. the upstream group. NR, non-risk; SNP, single nucleotide polymorphism.