Table I.
Primer nucleotide sequences.
| Gene | Primer nucleotide sequences (5′ to 3′) | Product size (bp) |
|---|---|---|
| GAPDH | F: GCACCGTCAAGGCTGAGAAC | 138 |
| R: TGGTGAAGACGCCAGTGGA | ||
| GLUT1 | F: GCCTGAAGTCGCACAGTGAATAA | 145 |
| R: GCTCATTGGGCCCATACAAAG | ||
| HK2 | F: CTCAACCATGACCAAGTGCAGAA | 97 |
| R: CCTTGCGGAACCGCTTAGAG | ||
| PFKM | F: GCCAGTCTAATTGCCGTTCC | 212 |
| R: TACCAACTCGAACCACAGCC | ||
| PKM2 | F: CCACTTGCAATTATTTGAGGAA | 148 |
| R: GTGAGCAGACCTGCCAGACT | ||
| LDHA | F: ATCTTGACCTACGTGGCTTGGA | 180 |
| R: CCATACAGGCACACTGGAATCTC | ||
| MCT1 | F: TTATCCTGCCACACCAGCAG | 352 |
| R: TGCTGTCACACACAGACACA | ||
| MCT4 | F: ATTGGCCTGGTGCTGCTGATG | 243 |
| R: CGAGTCTGCAGGAGGCTTGTG | ||
| IL-1β | F: TCGCCAGTGAAATGATGGCTTA | 197 |
| R: GTCCATGGCCACAACAACTGA |
GLUT1, glucose transporter 1; HK2, hexokinase 2; PFKM, phosphofructokinase, muscle; PKM2, pyruvate kinase M2; LDHA, lactate dehydrogenase; MCT, mono-carboxylate transporter; IL-1β, interleukin-1β; F, forward; R, reverse.