Table II.

Primers for the RT-qPCR detection of NSCs.

Names	Primer sequences
NESTIN	F: 5′-CCCCTTGCCTAATACCCTTGA-3′
	R: 5′-GCCTCAGACATAGGTGGGATG-3′
PAX6	F: 5′-GTTGTGTGAGTAAAATTCTGGGC-3′
	R: 5′-GAGTCGCCACTCTTGGCTTA-3′
BLBP	F: 5′-CGCAACCTGGAAGCTGACA-3′
	R: 5′-GCCCAGAGCTTTCATGTACTCA-3′
ZFP42	F: 5′-CCGGGATGAAAGTGAGATTAGC-3′
	R: 5′-TCACCTCGTATGATGCACTC-3′
GAPDH	F: 5′-AGGTCGGTGTGAACGGATTTG-3′
	R: 5′-GGGGTCGTTGATGGCAACA-3′

The GAPDH primer pair used was as listed in Table I. F, forward; R, reverse; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NSC, neural stem cell; PAX6, paired box 6; BLBP, brain lipid-binding protein.