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. 2017 Dec 12;6:e29905. doi: 10.7554/eLife.29905

Figure 2. Muscle fibers are excessively long in intercostal, levator auris, and diaphragm muscles in adult Abl2 mutant mice.

Whole mounts of adult muscle were stained with antibodies to myosin heavy chain (MHC) and GFP. Cross-sections of muscle were stained with antibodies to Laminin. (A) Intercostal muscle fibers normally extend from one rib to the adjacent rib (arrows) but extend and cross over one or more ribs in Abl2 mutant mice. (B) Muscle fibers in the levator auris muscle appear wavy in Abl2 mutant mice. Moreover, the midline tendon, marked by Scx::GFP, is reduced in size (double headed arrows). (C) Muscle fibers in the diaphragm muscle remain longer and the central tendon is reduced in size in adult Abl2 mutant mice. (D) The cross-sectional area of myofibers in adult, 8 week old, Abl2−/− mice is normal. Scale bar is 250 µm in A and B, and 50 µm in D.

Figure 2.

Figure 2—figure supplement 1. Limb muscles appear normal in muscle length and cross-sectional area.

Figure 2—figure supplement 1.

Hind limbs were fixed in 4% PFA, and tibialis anterior (TA) muscles were dissected at full length from three wild type and 3 Abl2−/− mice. Sections were stained with antibodies to Laminin to outline the muscle fibers. The length and cross-sectional area of Abl2−/− TA muscles are normal. The means ± SEMs from the left TA muscles of 3–4 mice in each group are shown. Scale bar is 50 µm.