Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| gene (Mus musculus) | Abl2 | The Jackson Laboratory | RRID:MGI:2653897 | |
| gene (Mus musculus) | Myod iCre | The Jackson Laboratory | RRID:IMSR_JAX:014140 | |
| gene (Mus musculus) | Mck-Cre | The Jackson Laboratory | RRID:IMSR_JAX:006475 | |
| gene (Mus musculus) | Pax3 Cre | The Jackson Laboratory | RRID:IMSR_JAX:005549 | |
| gene (Mus musculus) | Rosa 26 LacZ | The Jackson Laboratory | RRID:IMSR_JAX:003474 | |
| gene (Mus musculus) | Scx::Cre | MGI:5317938 | n/a | Ronen Schweitzer lab |
| gene (Mus musculus) | Scx::GFP | PMID: 17497702 | n/a | Ronen Schweitzer lab |
| strain, strain background (Mus musculus) |
C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX000664 | |
| genetic reagent (Mus musculus) |
Targeting Vector: Abl2tm1a(EUCOMM)Hmgu |
EUCOMM | RRID:SCR_003104 | European Conditional Mouse Mutagenesis Program |
| cell line (Mus musculus) | C2C12 skeletal muscle cells | Burden lab/ATCC | Cat# CRL-1772, RRID:CVCL_0188 | |
| antibody | Rabbit anti-Abl2 | Proteintech Group | Cat# 17693–1-AP RRID:AB_2289025 |
1:1000 |
| antibody | Goat anti-Arg (C-20) | Santa Cruz Biotechnology | Cat# sc-6356 RRID:AB_2221106 | 1:200 |
| antibody | Rabbit anti-β-Actin (13E5) | Cell Signaling Technology | Cat # 4970 | 1:1000 |
| antibody | Mouse anti-Myosin (MY-32) | Sigma-Aldrich | Cat# M7523 RRID:AB_260649 | 1:1000 |
| antibody | Chicken anti-GFP | Abcam | Cat# ab92456 RRID:AB_10561923 | 1:3000 |
| antibody | Mouse anti-Myosin (NOQ7.5.4D) |
Sigma-Aldrich | Cat# M8421 RRID:AB_477248 | 1:1000 |
| antibody | Rabbit anti-Laminin | Sigma-Aldrich | Cat# L9393 RRID:AB_477163 | 1:1000 |
| antibody | Mouse anti-Pax7 | Santa Cruz Biotechnology | Cat# sc-81648 RRID:AB_2159836 | 1:500 (bioreactor) |
| antibody | Rabbit anti-MyoD C-20 | Santa Cruz Biotechnology | Cat# sc-304 RRID:AB_631992 | 1:1000 |
| antibody | Mouse anti-MyoD (5.8A) | ThermoFisher Scientific | Cat# MA1-21816 RRID:AB_560242 | 1:1000 |
| antibody | Donkey anti-mouse (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 594 |
ThermoFisher Scientific | Cat# A-21203 RRID:AB_2535789 | 1:2000 |
| antibody | Donkey anti-rabbit (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 594 |
ThermoFisher Scientific | Cat# A-21207 also A21207 RRID:AB_141637 |
1:2000 |
| antibody | Donkey anti-mouse (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 488 |
ThermoFisher Scientific | Cat# A-21202 RRID:AB_141607 | 1:2000 |
| antibody | Donkey anti-rabbit (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 488 |
ThermoFisher Scientific | Cat# A-21206 also A21206 RRID:AB_2535792 |
1:2000 |
| antibody | Goat anti-Rat IgG (H + L) Cross-adsorbed Secondary Antibody Alexa Fluor 488 |
ThermoFisher Scientific | Cat# A-11006 also A11006 RRID:AB_2534074 |
1:2000 |
| commercial assay or kit | Click-iT Plus EdU Alexa Fluor 488 Imaging Kit |
ThermoFisher Scientific | Cat# C10637 | |
| software, algorithm | Prism 7.0 | http://www.graphpad.com/scientific-software/prism/ | RRID:SCR_002798 |