Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
strains are listed in Supplementary file 5 | ||||
primers are listed in Supplementary file 4 | ||||
genetic reagent (Plasmid) | pDZ274 | Addgene plasmid # 45929 | ||
genetic reagent (Plasmid) | pDZ415 | Addgene plasmid # 45162 | ||
antibody | anti-DIG-POD | Roche | RRID:AB_514500 | 1/750 in PBTB |
antibody | anti-HA | Covance | RRID:AB_2314672 | 1/250 FISH-IF, 1/1,000 WB |
antibody | anti-GFP | Roche | RRID:AB_390913 | 1/250 |
antibody | goat anti-mouse-IgG-Alexa 488 | Invitrogen | RRID:AB_2534069 | 1/400 in PBS |
antibody | anti-Tpi1 | LSBio | RRID:AB_11132833 | 1/1,000 |
antibody | anti-myc | Sigma-Aldrich | RRID:AB_439694 | 1/1,000 |
antibody | anti-Pgk1 | Invitrogen | RRID:AB_221541 | 1/1,000 |