Figure 8. The evolved repression by Bab for cis-regulatory regions 5’ of yellow.

(A–A’’) Comparison of the levels of EGFP-reporter expression in the male A5 and A6 segments driven by the Wing Body Element of D. melanogaster. (B–B’’) Comparison of the levels of EGFP-reporter expression in the male A5 and A6 segments driven by the Wing Body Element of D. malerkotliana. (C–C’’) Comparison of the levels of EGFP-reporter expression in the male A5 and A6 segments driven by the Wing Body Element of D. pseudoobscura. (D–D’’) Comparison of the levels of EGFP-reporter expression in the male A5 and A6 segments driven by the 5’ non-coding region of D. willistoni yellow. For each comparison, the level of EGFP expression are expressed as the percentage of the mean ±SEM for samples in which the Bab1-DBM was expressed. (A–D) Ectopic expression of the Bab1-DBM in the y-GAL4 pattern. (A’–D’) Ectopic expression of Bab1 in the y-GAL4 pattern. (A’’–D’’) Ectopic expression of Bab2 in the y-GAL4 pattern.

Figure 8—figure supplement 1. Orthologous regulatory regions 5’ of the yellow gene differ in their responsiveness to bab.

Scatter plots of the pixel intensity statistics obtained for the EGFP reporter expression occurring in the dorsal abdominal epidermis of the A5 and A6 segments and the A3 segment. For each condition (a reporter transgene with expression driven by a yellow CRE and an ectopically expressed bab open reading frame), expression was measured for five replicate male specimens, except for the D. pseudoobscura y5’ sequence in the presence of ectopic bab1 DBM expression (n = 4), for which the mean is shown as a horizontal black bar. All specimens used were at the developmental stage of ~85 hr after puparium formation for growth at 25°C.