Table 2. Design of small interfering RNA output for the bab2 ORF.
siRNA _id |
Position | SS sequence (Passenger) | AS sequence (Guide) | Corrected score |
---|---|---|---|---|
6 | 16 | GAUUGUGGACUUUGAAAUAAA | UAUUUCAAAGUCCACAAUCUG | 98.1 |
12 | 279 | CGGAGCUGGUGAAGUCCAAGG | UUGGACUUCACCAGCUCCGUU | 94.5 |
20 | 51 | GCGAAAUCGAUCAGUUCGAGG | UCGAACUGAUCGAUUUCGCCG | 94.4 |
18 | 155 | AGAAAGUACUCACCCGAAAGG | UUUCGGGUGAGUACUUUCUGU | 93.6 |
19 | 202 | AAGUGAGGUGGUUGAUCAAAU | UUGAUCAACCACCUCACUUGG | 92.5 |
23 | 241 | CGUUGGAGAAGUCAAGUCACC | UGACUUGACUUCUCCAACGCU | 92.3 |
42 | 1 | GGACAUGACCAAACAGAUUGU | AAUCUGUUUGGUCAUGUCCAU | 91.7 |
43 | 14 | CAGAUUGUGGACUUUGAAAUA | UUUCAAAGUCCACAAUCUGUU | 91.6 |
45 | 63 | AGUUCGAGGCGAGUGACUACA | UAGUCACUCGCCUCGAACUGA | 91.4 |
40 | 154 | CAGAAAGUACUCACCCGAAAG | UUCGGGUGAGUACUUUCUGUU | 90.7 |
49 | 13 | ACAGAUUGUGGACUUUGAAAU | UUCAAAGUCCACAAUCUGUUU | 90.7 |
28 | 306 | CGAUGAACGACCAAGCUUUGA | AAAGCUUGGUCGUUCAUCGGA | 90.6 |
46 | 140 | CUAGAGGACCAGAACAGAAAG | UUCUGUUCUGGUCCUCUAGAU | 90.4 |
13 | 625 | GACCAAUGUCUUUGACGAACU | UUCGUCAAAGACAUUGGUCAG | 90.3 |
55 | 12 | AACAGAUUGUGGACUUUGAAA | UCAAAGUCCACAAUCUGUUUG | 90.3 |
38 | 297 | AGGCGAGUCCGAUGAACGACC | UCGUUCAUCGGACUCGCCUUG | 89.8 |
27 | 443 | CAGCCUCAACCAAAUCUUAAG | UAAGAUUUGGUUGAGGCUGUG | 89.6 |
10 | 822 | UGGUGGAGUUCAUGUACAAGG | UUGUACAUGAACUCCACCAGG | 88.9 |
4 | 1099 | GGACUUGAAUCAGCGACAAAG | UUGUCGCUGAUUCAAGUCCAA | 88.8 |