Table 3. Oligonucleotides used to make Scan Mutant four region gel shift probes.

Probe	Sequence (5’ to 3’)	Oligo name
SM4 Region 1	ATTCTTTAATTTGTATTTTAATATT	yBE 4i1 Top
	AATATTAAAATACAAATTAAAGAAT	yBE 4i1 Bottom
SM4 Region 2	ATATTTTGAGAGGTTTTCCTTATTTAAAGT	yBE 4i2 Top
	ACTTTAAATAAGGAAAACCTCTCAAAATAT	yBE 4i2 Bottom
SM4 Region 3	AAAGTGTAGATTATTGAGGATTAAT	yBE 4i3 Top
	ATTAATCCTCAATAATCTACACTTT	yBE 4i3 Bottom
SM4 Region 3 Scan Mutant	cAcGgGgAtAgTcTgGcGtAgTcAg	y4i3 T Scrm
	cTgAcTaCgCcAgAcTaTcCcCgTg	y4i3 B Scrm
Region 3 TA > GA	AAAGTGgAGATgATTGAGGATgAAT	yBE 4i3 TA > GA Top
	ATTcATCCTCAATcATCTcCACTTT	yBE 4i3 TA > GA Bottom
SM4 Region 3 sub1	gggCgggCgATTATTGAGGATTAAT	y4i3 sub1 T
	ATTAATCCTCAATAATcGgggGccc	y4i3 sub1 B
SM4 Region 3 sub2	AAAGTgggCgggCgTGAGGATTAAT	y4i3 sub2 T
	ATTAATCCTCAcGcccGcccACTTT	y4i3 sub2 B
SM4 Region 3 sub3	AAAGTGTAGAgggCgggCgATTAAT	y4i3 sub3 T
	ATTAATcGcccGcccTCTACACTTT	y4i3 sub3 B
SM4 Region 3 sub4	AAAGTGTAGATTATTGgggCgggCg	y4i3 sub4 T
	cGcccGcccCAATAATCTACACTTT	y4i3 sub4 B