Table 5. Oligonucleotides for cloning bab1 and bab2 shRNAs into NheI and EcoRI sites of pattB-NE3 vector.
| siRNA name and sequence | Oligo name | Oligo sequence (5’ – 3’) |
|---|---|---|
| bab1 siRNA 3 TTCGTCTGATAGTTGTTCCAG |
b1_3 Top b1_3 Bottom |
ctagcagtCTGGAACAACAATCAGACGTAtagttatattcaagcataTTCGTCTGATAGTTGTTCCAGgcg aattcgcCTGGAACAACTATCAGACGAAtatgcttgaatataactaTACGTCTGATTGTTGTTCCAGactg |
| bab1 siRNA 4 TACAGCATGACCTTGACTCTC |
b1_4 Top b1_4 bottom |
ctagcagtGAGAGTCAAGCTCATGCTGAAtagttatattcaagcataTACAGCATGACCTTGACTCTCgcg aattcgcGAGAGTCAAGGTCATGCTGTAtatgcttgaatataactaTTCAGCATGAGCTTGACTCTCactg |
| bab2 siRNA 16 TATTTCAAAGTCCACAATCTG |
b2_16 Top b2_16 Bottom |
ctagcagtCAGATTGTGGTCTTTGAAAAAtagttatattcaagcataTATTTCAAAGTCCACAATCTGgcg aattcgcCAGATTGTGGACTTTGAAATAtatgcttgaatataactaTTTTTCAAAGACCACAATCTGactg |
| bab2 siRNA 12 TTGGACTTCACCAGCTCCGTT |
b2_12 Top b2_12 Bottom |
ctagcagtAACGGAGCTGCTGAAGTCCTAtagttatattcaagcataTTGGACTTCACCAGCTCCGTTgcg aattcgcAACGGAGCTGGTGAAGTCCAAtatgcttgaatataactaTAGGACTTCAGCAGCTCCGTTactg |
| bab2 siRNA 20 TCGAACTGATCGATTTCGCCG |
b2_20 Top b2_20 Bottom |
ctagcagtCGGCGAAATCCATCAGTTCCAtagttatattcaagcataTCGAACTGATCGATTTCGCCGgcg aattcgcCGGCGAAATCGATCAGTTCGAtatgcttgaatataactaTGGAACTGATGGATTTCGCCGactg |