Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Lrp1flox/flox | PMID:9634821 | RRID:IMSR_JAX:012604 | |
| Genetic reagent (Mus musculus) | Olig2-Cre | PMID:18691547 | RRID:MMRRC_011103-UCD | |
| Genetic reagent (Mus musculus) | CAG-CreERTM | PMID:11944939 | RRID:IMSR_JAX:004682 | |
| Genetic reagent (Mus musculus) | Pdgfra-CreERTM | PMID: 21092857 | RRID:IMSR_JAX:018280 | |
| Sequence-based reagent (cRNA) | Pdgfra cRNA | PMID:24948802 | ||
| Sequence-based reagent (cRNA) | Plp1 cRNA | PMID:24948802 | ||
| Sequence-based reagent (cRNA) | Mag cRNA | PMID:22131434 | ||
| Sequence-based reagent (cRNA) | Mbp cRNA | this study | Based on Allen Brain Atlas |
|
| Antibody | anti-Digoxigenin-AP antibody |
Roche | #11093274910 | |
| Antibody | rabbit anti-Olig2 | Millipore | #AB9610, RRID:AB_570666 | |
| Antibody | rat anti-PDGFRα | BD Pharmingen | #558774, RRID:AB_397117 | |
| Antibody | rabbit anti-GFAP | DAKO | # A 0334, RRID:AB_10013482 | |
| Antibody | mouse anti-APC | Calbiochem | #OP80, Clone CC1, RRID:AB_2057371 |
|
| Antibody | rabbit anti-Caspr | PMID: 9118959 | RRID:AB_2572297 | |
| Antibody | mouse anti-Na Channel | PMID: 10460258 | K58/35 | |
| Antibody | rabbit anti-CNPase | Aves Labs | #27490 R12-2096 | |
| Antibody | mouse anti-MAG | Millipore | #MAB1567, RRID:AB_2137847 | |
| Antibody | rat anti-MBP | Millipore | #MAB386, RRID:AB_94975 | |
| Antibody | chicken anti-PLP | Aves Labs | #27592 | |
| Antibody | mouse anti-GFAP | Sigma | #G3893, RRID:AB_477010 | |
| Antibody | chicken anti-GFAP | Aves Labs | #GFAP | |
| Antibody | rabbit anti-NG2 | Millipore | #AB5320, RRID:AB_91789 | |
| Antibody | rabbit anti-LRP1β | Abcam | #ab92544, RRID:AB_2234877 | |
| Antibody | rabbit anti-PMP70 | Thermo | #PA1-650, RRID:AB_2219912 | |
| Antibody | mouse anti-βIII tubulin | Promega | #G7121, RRID:AB_430874 | |
| Antibody | mouse anti-β-actin | Sigma | #AC-15 A5441, RRID:AB_476744 |
|
| Antibody | rabbit anti-MAG | PMID: 27008179 | ||
| Antibody | rabbit anti-PLP | Abcam | #ab28486, RRID:AB_776593 | |
| Antibody | rat anti-PLP/DM20 | PMID: 27008179 | AA3 hybridoma | Wendy Macklin |
| Antibody | mouse anti-CNPase | Abcam | #ab6319, RRID:AB_2082593 | |
| Antibody | rabbit anti-PXMP3 (PEX2) | One world lab | #AP9179c | |
| Antibody | rabbit anti-SREBP2 | One world lab | #7855 | |
| Commercial assay or kit | Cholesterol/Cholesteryl Ester Quantitation Kit |
Chemicon | #428901 | |
| Commercial assay or kit | DCTM Protein Assay | Bio-Rad | #5000112 | |
| Commercial assay or kit | RNeasy Micro Kit | Qiagen | #74004 | |
| Chemical compound, drug | 10 mM dNTP mix | Promega | #C1141 | |
| Chemical compound, drug | 5X Green GoTaq Buffer | Promega | #M791A | |
| Chemical compound, drug | GoTaq DNA polymerase | Promega | #M3005 | |
| Chemical compound, drug | L-α-Lysophosphatidylcholine | Sigma | #L4129 | |
| Chemical compound, drug | Hoechst dye 33342 | Life technology | #H3570 | |
| Chemical compound, drug | ProLong Gold antifade reagent |
Life technology | #P36930 | |
| Chemical compound, drug | Fluoromyelin-Green | Life technology | #F34651 | |
| Chemical compound, drug | PDGF-AA | Peprotech | #100-13A | |
| Chemical compound, drug | Forskolin | Sigma | #F6886 | |
| Chemical compound, drug | CNTF | Peprotech | #450–02 | |
| Chemical compound, drug | NT-3 | Peprotech | #450–03 | |
| Chemical compound, drug | T3 | Sigma | #T6397 | |
| Chemical compound, drug | Cholesterol | Sigma | #C8667 | |
| Chemical compound, drug | Pioglitazone | Sigma | #E6910 | |
| Chemical compound, drug | Simvastatin | Sigma | #S6196 | |
| Chemical compound, drug | GW9662 | Sigma | #M6191 | |
| Chemical compound, drug | Filipin | Sigma | #F9765 | |
| Chemical compound, drug | Super Signal West Pico substrate |
Thermo | #34080 | |
| Chemical compound, drug | WesternSure PREMIUM Chemiluminescent Substrate |
LI-COR Biosciences | #926–95000 | |
| Chemical compound, drug | Super Signal West Femto substrate |
Thermo | #34095 | |
| Software, algorithm | Fiji | PMID: 22743772 | ||
| Software, algorithm | Axon pAlamp10.3 software |
Molecular Devices | ||
| Software, algorithm | Origin9.1 software | Origin Lab | ||
| Software, algorithm | Image Studio Lite Western Blot Analysis Software |
LI-COR Biosciences | ||
| Other | C-DiGit blot scanner | LI-COR Biosciences | #P/N 3600–00 | |
| Other | Multimode Plate Reader | Molecular Devices | #SpectraMax M5e | |
| Other | Stoelting stereotaxic instrument |
Stoelting | #51730D | |
| Other | Stoelting quintessential stereotaxic injector | Stoelting | #53311 |