Figure 5. Restoration of ABCA3 expression in AT2 cells after partial deletion.

(A) Representative confocal immunofluorescence for ABCA3 (white) and proSPC (red) in alveolar epithelial cells from control and Abca3-cKO mice treated with tamoxifen for 4 days with tissue harvest at 2 and 12 weeks. Scale bars: 50 μm. Inset cell scale bars: 5 μm. (B) ABCA3⁺proSPC⁺ epithelial cells shown in panel A were quantitated using Nikon Elements General Analysis software. Data represent ABCA3⁺proSPC⁺ cells, mean ± SEM, n = 3–4 mice/group, **P = 0.0075 as determined by 1-way ANOVA.