Figure 6. Nrf2 staining in 2 additional diabetic animal models.

Pancreatic sections from hyperglycemic male ZDF rats showed substantial immunostaining for Nrf2 (A, red fluorescence), in β cells (green fluorescence), whereas ZDF rats fed with the antioxidant Ebselen along with the high-fat diet for 6 weeks did not stain for Nrf2 (B). (C) Hyperglycemic WT db/db mice fed a high-fat diet showed increased immunoreactivity for Nrf2 in β cells, whereas db/db mice with β cell–specific overexpression of GPx-1 fed a high-fat diet showed no detectable staining for Nrf2 (D). Scale bars: 50 μm.