Figure 6. MDSC populations from the spleen and bone marrow are not affected by changes in tumor-secreted human IL-8.

Quantification of total CD11b⁺ cell, PMN-MDSC (Ly6G^hi/Ly6C^lo), and M-MDSC (Ly6G^lo/–/Ly6C^hi) populations from spleens (A) and bone marrow (C) of mice treated with control HBSS (C), docetaxel (D), HuMax-IL8 (H), and the combination of HuMax-IL8 and docetaxel (H+D). Data represent mean ± SEM; n = 5–8 mice per group. Differences between means were compared using the 1-way ANOVA test with Tukey’s multiple comparisons. (B and D) Representative FACS plots of Ly6G/Ly6C-positive populations gated on CD11b⁺ cells for each treatment group in spleen (B) or bone marrow (D) samples. Data are representative of 2 experiments.