Figure 7. Human primary myelofibrosis samples have increased β-arrestin2 expression versus normal marrow controls.

Primary human bone marrow samples from patients with primary myelofibrosis (PMF) and normal bone marrow controls were stained for β-arrestin2 (βarr2) utilizing A2CT antibody. A second slide per sample was stained using nonimmune IgG control, and immunofluorescence was quantified. βarr2 expression was normalized to IgG background and DAPI staining. (A) Primary human PMF samples had increased βarr2 expression versus normal marrow controls (*P = 0.048 by t test). All images were quantified in triplicate. (B) Representative images of normal human bone marrow and PMF samples. Original magnification, ×20.