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. 2017 Nov 2;2(21):e96094. doi: 10.1172/jci.insight.96094

Figure 3. MCD diet–induced hepatic inflammation is diminished by miR-141/200c deficiency.

Figure 3

(A) Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT),and (B) IL-6 were measured in overnight fasted serum of WT and miR-141/200c–/– mice fed the control diet (CD) or methionine and choline–deficient (MCD) diet (n = 5–8 mice per group). (C–F) qPCR analysis of hepatic genes involved in inflammation (Saa1, Orm1, IL-1β, TNFα, IL-4, F4/80, LyG6) and fibrosis (col1a1, TGFβ) (n = 5 mice per group). (G) Representative images of Masson’s trichrome staining and F4/80 immunohistochemistry to examine fibrosis and macrophage activation in WT MCD and miR-141/200c–/– MCD. Images were quantified by digital image analysis using ImageJ software in 6 randomly chosen fields from 3 individual mice per group. (H) qPCR analysis of hepatic genes associated with M1 (iNOS) or M2 (Arg1) macrophage phenotype in WT MCD and miR-141/200c–/– MCD mice (n = 5 mice per group). Data are represented as mean ± SEM. Differences between 2 groups were compared using a Student’s unpaired t test. For multiple groups, differences were compared using a one-way ANOVA followed by Newman-Keuls multiple comparisons test. *P < 0.05 and **P < 0.01 indicate statistical significance.