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. 2017 Nov 2;2(21):e96094. doi: 10.1172/jci.insight.96094

Figure 4. miR-141/200c deficiency polarizes macrophages toward the M2 antiinflammatory phenotype.

Figure 4

BM-derived macrophages (BMDMs) were generated from WT and miR-141/200c–/– mice and incubated with or without LPS (10 ng/ml) for 24 hours and then processed for RNA isolation (n = 2 independent experiments). qPCR analysis of mRNA expression of (A) M1 markers (iNOS, IL-6, and IL-1β), (B) M1 regulators (HIF1α and NF-κβ), (C) M2 markers (Arg1 and IL-10), and (D) M2 regulators (KLF4, C/EBPβ, and STAT3). Data are represented as mean ± SEM. Differences between multiple groups were compared using a one-way ANOVA followed by Newman-Keuls multiple comparisons test. *P < 0.05 and **P < 0.01 indicate statistical significance.