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. 2017 Dec 7;2(23):e95128. doi: 10.1172/jci.insight.95128

Figure 7. 2DG decreases human NK cell cytotoxicity.

Figure 7

Human NK cells were isolated from healthy donors, enriched using RosetteSep, and cryopreserved. Cells were later thawed and activated in 100 ng/ml human IL-15 for 24 hours in various concentrations of 2DG. (A) NK cells were washed and cocultured with K562 targets for 4 hours at various E:T ratios; representative killing assay shown (n = technical duplicate, representative of 6 donors across 4 experiments, mean with replicates shown). (B and C) NK cells from control conditions and 20–40 mM 2DG were cultured at a 2:1 ratio with K562 cells and imaged after 40 minutes. (B) Quantification of actin accumulation by area × intensity of synapse – (NK + target) (n = 41–43 conjugates/group, 1 experiment). (C) Quantification of distance from the microtubule organizing center (MTOC) to synapse in μm (n = 67–61 conjugates/group, 2 experiments). Gray boxes show median, 25th, and 75th percentiles. Neither B nor C was significant by 2-tailed t test or Kolmogorov-Smirnov test.