Skip to main content
. 2017 Nov 2;2(21):e81836. doi: 10.1172/jci.insight.81836

Figure 5. Multimerized hen egg lysozyme embedded within the glomerular basement membrane following i.v. injection.

Figure 5

(A) The production of multimerized hen egg lysozyme (HEL). Monomeric HEL was biotinylated and then complexed with avidin to form multimers. (B) Representative Coomassie blue–stained SDS-PAGE of monomeric and multimerized HEL demonstrated multimerized complexes of at least 120 kDa were formed. (C) Immunofluorescence microscopy images (green = HEL complex, blue = DAPI) of frozen sections from kidneys of mice injected with multimerized HEL (left: 30 minutes after injection, center: 7 days after injection) or monomeric HEL (right: 30 minutes after injection) and stained with anti-HEL antibody. Monomeric HEL was not visible in glomeruli, while multimerized HEL was found in the glomeruli up to 7 days after injection. Glomeruli are outlined in circles. Original magnification, ×20. Data are representative of 3 independent experiments.