Figure 7. ILC2 polarization is impaired in mice lacking Pla2g10.

Gating was performed to isolate lineage negative (Lin^–), CD45⁺ leukocytes (Lin^–CD45⁺, see Supplemental Figure 3). Further gating on the Lin^–CD45⁺ leukocyte population was performed to isolate (A) ST2⁺ cells and (B) ST2⁺CD127⁺ cells from WT and Pla2g10^–/– mice exposed to saline or HDM. (C) Additional analysis revealed no significant differences in ST2⁺CD127⁺Sca1⁺ cells between WT and Pla2g10^–/– mice (n = 3 mice/group). Mean ± SEM, 2-way ANOVA with uncorrected Fisher’s LSD.