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. 2017 Nov 2;2(21):e94929. doi: 10.1172/jci.insight.94929

Figure 8. Lack of Pla2g10 alters mast cell activation.

Figure 8

(A) Lung tissue mast cells were visualized and enumerated using toluidine blue staining in mice exposed to saline or HDM (n = 6 mice for Sal and 8 mice for HDM). Mean ± SEM, Mann-Whitney test. (B) The expression of Il13 in WT BMMCs was evaluated in response to 4-hour stimulation with IL-33 (10 ng/ml), IL-13 (10 ng/ml), or HDM (250 μg/ml) (n = 4/group for Sal, IL-33, and IL-13 and 2 for HDM). (C) WT and Pla2g10–/– BMMC lysates were analyzed for sPLA2-X protein content via Western blot. WT and Pla2g10–/– BMMCs were stimulated with IL-33, IL-25, and TSLP (all at 10 ng/ml), and expression of Il13 was evaluated (n = 6–8/group for Sal and IL-33, 2–4/group for IL-25 and TSLP). Mean ± SEM, 1-way ANOVA with uncorrected Fisher’s LSD.