Figure 1. Hepatic deletion of ChREBP leads to blunted effects of fructose feeding on body weight and hepatic gene expression.

(A) Western blot for carbohydrate responsive element–binding protein (ChREBP) protein in liver and perigonadal (PG) adipose tissue whole-cell lysates of control (CTL) and liver-specific ChREBP-KO (LiChKO) mice. (B) Eight-week-old female control and LiChKO mice were fasted for 6 hours, and then gavaged with water or fructose and sacrificed 100 minutes later and hepatic and intestinal gene expression was measured (n = 4 per group). (C and D) Control and LiChKO male mice were fed chow or high-fructose diet (HFrD) starting at 7 weeks of age (n = 5 per group) and body weight was measured weekly. (D) Body composition (EchoMRI) was measured after 8 weeks. Body weights are presented as mean ± SEM. All other data are presented as box-and-whisker plots where the line in the box indicates the median, the box extends from the 25th to 75th percentiles, and the whiskers indicate the minimal and maximal values. P values were obtained using 2-way ANOVA. *P < 0.05 compared between water and fructose within the same genotype; ^#P < 0.05 compared between different genotypes within the same treatment group.