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. 2017 Dec 21;2(24):e96455. doi: 10.1172/jci.insight.96455

Figure 1. Accepting lung allografts are infiltrated by recipient-derived iNOS+ cells.

Figure 1

(A) Quantitative RT-PCR revealed a significant increase in iNOS transcript levels 7 days after transplantation of BALB/c lungs to B6 recipients with CSB immunosuppression. (B) Flow cytometric analysis demonstrated that lung allografts are infiltrated by iNOS+ hematopoietic cells (representative of 8 separate transplants). (C) Time-course analysis demonstrated a gradual increase in iNOS+ cells, which decrease to baseline levels 1 month after transplantation (representative of at least 4 transplants per time point). (D) Transplantation of a BALB/cCD45.2+ lung grafts to a B6CD45.1+ recipient demonstrated that all iNOS+ graft-infiltrating cells are of recipient origin (representative of 3 separate transplants). *P <.05, **P < 0.01. Comparison by Friedman’s test was used for paired data, while the Kruskal-Wallis test was used for the unpaired group of observations. Post-hoc analysis of differences and comparison of differences between pairs of data were performed with the Wilcoxon rank test and the Mann-Whitney U test for paired and unpaired observations, respectively.