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. 2017 Nov 16;2(22):e92902. doi: 10.1172/jci.insight.92902

Figure 5. Characterization of CD169 as a marker for resident macrophages in the liver.

Figure 5

(A) F4/80hi resident and CD11bhi recruited macrophages were sorted from hepatic NPCs of wild-type mice fed SD. mRNA expression of Emr1, Clec4f, Siglec1 (CD169), Itgam, Ccr2, and Ly6c1 was analyzed by quantitative real-time PCR. Data represent mean ± SEM. **P < 0.01 (2-tailed unpaired Student’s t test). n = 5. (B) Immunofluorescent staining for Clec4f and CD169 of livers of wild-type mice fed SD (WT/SD) and MC4R-KO mice fed WD (MC/WD) for 20 weeks. Immunofluorescent staining for CD169 and type I collagen in MC/WD and WT/SD for 20 weeks (C) and the inducible NASH model (D). Veh, WD-fed MC4R-KO mice 7 days after vehicle (olive oil) injection; CCl4_day7, WD-fed MC4R 7 days after CCl4 injection. Scale bar: 50 μm.