Figure 7. Persistent synaptic strength alterations were rescued by Pimozide in the SOD1^G37R EDL.

Paired experimental results showing synaptic strength before and after a 30- to 45-min bath application of pimozide 5 μM, on the same neuromuscular junction. Pimozide-enhancing effect on synaptic transmission was clearly demonstrated at the presymptomatic stage as well as disease onset on evoked endplate potential (EPP) amplitude (A and E), quantal content (B and F), miniature endplate potential frequency (mEPP frequency) (C and G), and mEPP amplitude (D and H) before (square) and after pimozide bath application in SOD1 neuromuscular junctions (triangle). Quantal content was obtained by dividing the mean EPP of the first EPPs, including failures, by the mean mEPP amplitude.*P < 0.05, **P < 0.01, ***P < 0.001. All data values are given as mean ± SEM. Significance was determined using Students 2-tailed t test.