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. 2017 Nov 16;2(22):e97180. doi: 10.1172/jci.insight.97180

Figure 6. BINS algorithm.

Figure 6

(A) Raw data of an embryonic zebrafish heart at 5 dpf. (B) Adaptive histogram equalization by CLAHE. (C) Contrast adjustment. (D) Range filter. (E) Binary by imbinarize. (F) Filtering the largest area. (G) Filling holes. (H) Subtracting outside boundary by inside boundary after localizing and filling the contour. (I) Pixel intensity values for A–H measured along the red dashed line in A. BINS, batch intensity normalized segmentation; CLAHE, contrast-limited adaptive histogram equalization.