Figure 6. Clonal relationships between CSF naive, USM, and CD27^hi B cells and other CSF or peripheral blood B cells suggest recruitment of antigen-specific and nonspecific B cells.

CSF B cell subsets are in blue type; PB subsets are in red type. Numbers represent number of clusters identified containing clonally related Ig-VH from both connected subsets; in the CD27^hi column, blue numbers represent CSF-restricted Ig-VH clusters, red numbers represent clusters comprising CSF and PB subsets. For simplicity, only the direct connections between the CSF subset of interest (bold blue type) and other subsets are shown. See Supplemental Figure 3 for an example detailed overview of clonal connections between CSF-naive B cells and other CSF or PB subsets (patient 56414). For comprehensive information on clonal relationships between CSF B cells and other subsets see Supplemental Table 4. n.a., not available, i.e., CSF subset not obtained flow cytometry sorting; N, naive B cells; USM, unswitched-memory B cells; DN, double-negative B cells; SM, switched-memory B cells; PC, plasma cells/plasmablasts.