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. 2017 Nov 16;2(22):e97222. doi: 10.1172/jci.insight.97222

Figure 5. Allosteric activity of W-18 on agonist stimulated β-arrestin recruitment at opioid receptors.

Figure 5

The arrestin recruitment activity was determined in the GPCR Tango assays, as outlined in the Methods. W-18 failed to modify the effect of DADLE at DOR (A), dynorphin A at KOR (B), dermorphin at MOR (C), or nociceptin at NOP (D) receptors. Normalized results represent mean ± SEM of 3 independent experiments, each performed in triplicate (0% activity for vehicle and 100% for reference activity), and were analyzed in Prism.