| Primer pair | Primer type | Primer sequence |
| 28KJ/28B | Plant | GGC GGT AAA TTC CGT CC/ |
| CGT CCG TGT TTC AAG ACG | ||
| ITS1/ ITS5.8 | Fungal | TCC GTA GGT GAA CCT GCG G/ |
| GAG ATC CGT TGT TGA AAG TT | ||
| 16s forward/ reverse | Nested bacterial | 5’- ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCTACGGGNGGCWGCAG-3’/ |
| 5’- GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGACTACHVGGGTATCTAATCC-3’ | ||
| ITS1F primer/ ITS4 | Nested fungal | 5’- ACACTCTTTCCCTACACGACGCTCTTCCGATCTTTCGTAGGTGAACCTGCGG - 3’ |
| 5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTTCCTCCGCTTATTGATATGC - 3’ | ||
| Adapter primers | 5’-AATGATACGGCGACCACCGAGATCTACAC[55555555] ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ | |
| 5’-CAAGCAGAAGACGGCATACGAGAT[77777777] GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT -3’ |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.